TFF1 anticorps (C-Term)

N° du produit ABIN6251839

L’anticorps Souris Monoclonal anti-TFF1 a été validé pour WB, IHC, IF et FACS. Il convient pour détecter TFF1 dans des échantillons de Humain et Cynomolgus.

Aperçu rapide pour TFF1 anticorps (C-Term) (ABIN6251839)

Antigène: TFF1 (Trefoil Factor 1 (TFF1))

Reactivité: Humain, Cynomolgus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TFF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: GE2

Détail du produit anti-TFF1 anticorps

Épitope: C-Term

Purification: Protein G affinity chromatography

Immunogène: Amino acids CFDDTVRGVPWCFYPNTIDVPPEEECEF (57-84) from the C-terminus of human Estrogen inducible protein pS2 was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues REQUIRES digestion with Pronase E at 1 mg/mL in PBS for 15 min at 37 °C. Heat Induced Epitope Retrieval is not suitable for this antibody.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur TFF1

Antigène: TFF1 (Trefoil Factor 1 (TFF1))

Autre désignation: Estrogen Inducible Protein pS2

Sujet: This antibody recognizes a polypeptide of ~6.5  kDa, identified as Estrogen Inducible Protein pS2. The antibody's epitope is localized between amino acids 57-84. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. Estrogen Inducible Protein pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60 % of breast carcinomas are positive for estrogen inducible protein pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. It is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that estrogen inducible protein pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.

ID gène: 7031

UniProt: P04155

Pathways: EGFR Signaling Pathway